The announcement said Wyatt's contract would not be renewed and the "change is effective immediately."
Athletic Director Lynn W. Thompson said it would be a "tremendous challenge" to find a replacement for Wyatt.
The Wildcats were 90-54, including 5-6 this season, under Wyatt, who became coach of the team Nov. 27, 1996.
"Today marks the end of an era in Wildcat football that many have enjoyed and celebrated," Thompson said.
Bethune-Cookman teams compete in the Mid-Eastern Athletic Conference.
The Wildcats were 11-2 and won the MEAC championship in 2002, their best season under Wyatt, who was a player at Bethune-Cookman and played in the NFL with the Buffalo Bills, Oakland Raiders and the Houston Oilers, who have since become the Tennessee Titans.
Copyright 2009 by United Press International.
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